Results

Publications

Antimicrobial Effect of Rana Ridibunda Skin Gland Peptides against Multidrug Resistant Pathogens

Abstract

Amphibian skin glands have gained a lot of attention for secreting a wide variety of bioactive chemical compounds, with notable significance in pharmacology and therapeutics. The continuous emergence of bacterial and fungal strains with varying degrees of resistance to common antimicrobial agents justifies the urgent need to develop new antimicrobials that can meet this challenge. In the present study, the protein secretions from the skin glands of the frog Rana ridibunda were collected. and tested against four different microorganisms, namely, methicilinresistant Staphylococcus aureus, ESBL Klebsiella pneumonia, ESBL Esherichia coli and Candida albicans. Inhibition zone diameters of 17, 15, 15, and 31 mm with minimum inhibitory concentration of 1.18, 4.73, 4.73 and 0.29 mg/ml were observed for methicilin-resistant Staphylococcus aureus, ESBL Klebsiella pneumonia, ESBL Esherichia coli and Candida albicans, respectively. Electron microscopic study of microbial cells treated with the peptidyl secretions demonstrated direct bactericidal and fungicidal capabilities. This promising data suggested that the antimicrobial skin gland peptides of the Lebanese frog Rana ridibunda are active against all the tested microbial strains and should provide basis for the development of new therapeutic agents against multidrug resistant pathogens.

Author(s)

Moussad E.A.

Coauthor(s)

El-Dakdouki M.H., Olama Z., Haj-Moussa A.A.

Journal/Conference Information

International Journal of Microbiology and Applied Science,2015, 4, 62-74