Hasbani River in South Lebanon has very high total and fecal coliforms levels above the international accepted limits. The direct discharge of contaminants rendered the water river not suitable for drinking and recreational activities. The present study was managed to study the water quality of the river during January, August and November 2013. Water samples were analyzed for the following parameters: pH, electrical conductivity, total dissolved solids, biochemical oxygen demand, inorganic chemicals (PO4, NO3, SO4, Na, K, Ca, Mg, Cl), trace metals (Zn, Pb, Cu, Co, Cd), pesticides, heterotrophic bacteria, total and fecal coliforms, Salmonella sp. and Shigella sp.. The presence of all these chemical and biological contaminants in the study area proved the high pollution. These toxins were concentrated within aquatic food chains and caused detrimental effects to the aquatic life.