Periodontal therapy is one of the etiological factors of dentine hypersensitivity (DH). This study aimed to evaluate the efficacy of two in-office desensitizing agents 8 % Arginine-CaCO3 and 1.23 NaF varnish on reducing DH that affects patients with chronic periodontitis after routine periodontal treatment. Study design: Forty chronic periodontitis patients with history of dentine hypersensitivity after periodontal therapy were included in this study, and randomly divided into two groups: group-1, who received 8%Arginine-CaCO3 and group-2, who received 1.23%NaF varnish. The clinical indices were recorded at first visit. Dentine hypersensitivity was evaluated by using tactile, air-blast, and thermal stimuli. The subject’s response was recorded at baseline, one month and 3 month after the application. Results and conclusions: The results were statistically analyzed, and it was found that 8% Arginine-CaCO3 treatment was more effective than 1.23% NaFvarnish at time intervals. Sensitivity score differences between the groups were statistically significant at one and three months. The 8% Arginine-CaCO3 group exhibited statistically significant reduction in dentine hypersensitivity on three stimuli at baseline to one and three months. It was concluded that 8% Arginine-CaCO3 is more effective than 1.23% NaF varnish in reduction of patients’ pain in chronic periodontitis patients. KEY WORDS: Chronic periodontitis, desensitizing agent, dentine hypersensitivity