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Effect of Different Irrigation Regimens on Enterococcus faecalis Elimination from Infected Root Canals (An In-Vitro Comparative Study)


Microorganisms harbored in the complexities of root canal systems might lead to endodontic failures and development of apical periodontitis. Enterococcus faecalis is the most common isolated bacteria in these cases. Aim: This study was conducted to compare the efficiency of four irrigation regimens on the elimination of an inoculated strain of E. faecalis. Materials and Methods: Forty single rooted extracted premolars were inoculated with a standard strain of Enterococcus faecalis (ATCC 29212) and incubated at 37˚C for two weeks to allow infection of the dentinal tubules. The bacterial suspension was replaced with a new one every 48 hours. The teeth were then divided into four groups of n=10 each, to apply irrigation regimens as follows: Group i: irrigation with normal saline, Group ii: syringe irrigation (NaOCl 2.625%), Group iii: NaOCl 2.625%+EndoActivator and Group iv: NaOCl 2.625%+diode laser. Samples were collected at baseline and after irrigation. Colony-forming unit counts were performed. For baseline count of E. faecalis, differences in means between the groups were tested using the one way ANOVA F test. For the count of E. faecalis after the irrigation, differences in means was assessed using the Kruskal Wallis test. Results: The mean values of % of E. faecalis killed were respectively 99.2 ± 2.53% in group iv, 98.3± 5.34% in group iii, 29.0 ± 5.46% in group ii and 4.77 ± 0.78%) in group i. Conclusion: Maximum removal of E. faecalis strains was achieved by activating 2.625% sodium hypochlorite with the diode laser. Keywords Bacterial Eradication, Enterococcus faecalis, Syringe irrigation, EndoActivator, Diode laser.

Journal/Conference Information

BAU Journal - Health and Wellbeing,DOI: 000000, ISSN: 2617-1635, Volume: 4, Issue: 1, Pages Range: 1-8,